On June 22, 1564, French explorer René de Laudonnière (ca. 1529-1574) landed in Florida. Days later he established the settlement of Fort Caroline. The fort was situated near the mouth of the St. Johns River, known to the French as the River May, north of present-day Jacksonville. The French had previously explored the region during the expedition headed by Jean Ribault (1520-1565) in 1562.
Upon learning of Spanish ships landing south of Fort Caroline, the French launched a military expedition on September 10, 1565. A hurricane battered the French ships before they reached the upstart Spanish settlement of St. Augustine. The survivors came ashore several dozen miles south of their intended target.
Throughout September and October 1565, Spaniards under the command of Pedro Menéndez de Avilés (1519-1574) attacked French survivors returning to Fort Caroline. The Spanish assaults occurred near an inlet 15 miles south of St. Augustine, later named Matanzas (massacre in Spanish). The Fort Caroline Massacre, as the attack has come to be known, halted French colonization of Florida and ushered in a period of Spanish control over the peninsula that lasted until 1763.